|
 |
| Model | CPC80 | CPC240 | CPC1400 | CPC5400 |
| Rotational Speed Washing Speed Separation Speed |
0 ~ 2000 rpm (100 rpm steps) 300 rpm 1500 rpm |
0 ~ 1500 rpm (100 rpm steps) 300 rpm 1100 rpm |
0 ~ 1300 rpm (100 rpm steps) 300 rpm 1000 rpm |
|
| Flow Rate Washing Flow Separation Flow |
0.1 ~ 30 ml/min 20 ml/min 5 ml/min |
1 ~ 200 ml/min 80 ml/min 25 ml/min |
10 ~ 500 ml/min 200 ml/min 100 ml/min |
|
| Standard Valves | 4-way mode switching valve | 4-way mode switching valve 6-way sample injection valve |
||
| Display | LCD Display | |||
| Rotor Material | Polly-phenylenesulfide (PPS), Stainless Steel | Poly-chlorotrifluoroethylene (DAIFLON), Stainless Steel |
||
| Number of Disk | 4 pcs x 1 Pack | 12 pcs x 1 Pack | 18 pcs x 1 Pack | 13 pcs x 2 Packs |
| Rotor Dimension | 200 x 20 H mm |
200 x 80 H mm |
300 x 150 H mm |
300 x 380 H mm |
| Rotor Volume | 80 ml | 240 ml | 1400 ml | 5400 ml |
| Partition Cells | 712 | 2136 | 1296 | 1040 |
| Cell Length | 15 mm | 29 mm | 46 mm | |
| Centrifugal Radius | 82.5 mm (average) | 120 mm (average) | 111 mm (average) | |
| Estimated Separation/Run | 8 ml | 24 ml | 140 ml | 540 ml |
| Maximum Pressure | 60 kg/cm2 | |||
| Safety Device | The rotor is automatically stopped when its lid is opened | |||
| In/Out Line Tubing Size | 1/16" PEEK (0.75 ID x 1 mm OD) | 1/8" SUS x 2 mm OD | ||
| Rotor Net Weight | 5 kg | 7 kg | 25kg | 40 kg |
| Overall Dimension | 330 W x 475 D x 480 H mm | 420 W x 610 D x 500 H mm | 600 W x 810 D x 1060 H mm | |
| Net Weight | 40 kg | 43 kg | 70 kg | 290 kg |
| Power Source | AC 100V ~ 240V, 3A, 1 50/60 Hz |
AC 200 ~220V, 3A, 3 50/60 Hz |
||
| Customized sample separations prior to purchase
are performed by us at reasonable expense. * Specifications are subject to change without prior notice. * Patents for Rotor Joint in Japan and for Rotor in U.S.A. |
| Options Dedecated Controller, Semi-preparative Pump, Injector, UV-Vis Detector, Fraction Collector Specifications of the CPC Controller (Option)
Specification of the Solvent Delivery Pump (Option)
|
|
| High Performance Centrifugal Partition Chromatography, HPCPCTM, is a new technique of Liquid Chromatography, LC, which is support free, and which finds its place among the several Liquid Chromatographic methods as shown on the following chart ; |
 |
Around fifty years ago, the concept of partitioning
solutes between two liquids gave birth to
two cognate methods, one was the counter-current
distribution, another was the liquid liquid
partition chromatography. Thirty years ago,
Sanki Engineering Ltd. (now absorbed by System
Instruments Company Ltd.) opened the way
to high performance centrifugal partition
chromatography (HPCPCTM), which was taking the best of the two first
techniques, namely the versatility
of a true
liquid liquid process combined with
the quickness
and advanced technology of chromatography.
The HPCPCTM is gaining more and more interest as a semi-prep
and preparative scale chromatographic method.
The four main advantages of the HPCPCTM over its parent prepscale column chromatography
are : No loss of sample since both mobile and stationary phases
are liquids and can be collected for total
recovery. The volume ratio of stationary to mobile
phases is definitely much higher, which leads
to higher capacities and better resolution with no need of a high number of theoretical
plates. The extreme flexibility of biphasic systems (mixtures of two or three or four solvents),
which allows to modify the selectivity of
a system in order to get a pure compound,
in the HPCPCTM the polarities of both phases can be
smoothly modified. The reduced solvent consumption, ten times less than for preparative scale chromatography
for the same throughput, which is of interest
for environmental considerations. Separation
accomplished with laboratory HPCPCTM can be directly scaled up to production
scale HPCPCTM. Another major advantage is the extremely low price of the stationary
phase (solvents) compared to that of column
packings. Moreover, stationary can be refreshed
easily, and added materials like chiral selectors
or complexing agents can be recovered with
no loss. Several publications in international
journals bring valuable information.The new innovated HPCPCTM is getting more involved in many fields of chemistry, for purification of antibiotics, petides, tannins, saponins, lipids, drugs, ...... its future development will see the production of bigger HPCPCTM units, and it will incorporate crucial fields of chemistry, such as chiral separations. |
|
Superior to Conventional Preparative LC
| HPCPCTM is fast ! Since stationary phase solvents are retained in the partition channels by centrifugal force, high mobile phase flow rates may be used without appreciable loss of resolution. Great advantages over conventional preparative
LCSince a solid support is not used with HPCPCTM, irreversible retention of valuable sample components is completely eliminated, denaturation and decomposition, often encountered with conventional LC column packings, are virtually eliminated. And this is generally accomplished with retention of biological activity. Moreover, the capacity of an HPCPCTM column (rotor) is significantly greater than an HPLC column of the same total volume. Consequently, overload effects are rarely encountered with HPCPC. Purification of large quantities is routine, always with 100% material balance...there is no adsorption or irreversible retention. No columns or Packings to replaceThe problems of formation of voids, contamination of fractions with silica and with components of previous runs, and the cost of replacing expensive HPLC columns are gone. Fewer Theoretical Plates are NeededTo achieve a given level of resolution between two peaks with HPCPCTM than with HPLC, for instance, for a value of alpha (selictivity) = 1.2 and K (partition coefficient) = 1.... 185,000 T.P are needed to achieve baseline resolution (Rs of 1.5) with HPLC....whereas only 2,200 plates are needed to do the job with HPCPCTM. This is a direct consequence of the standard resolution equation for liquid chromatography. Normal phase and reversed phase chromatography
can be done in the same runUse dual mode HPCPCTM to accomplish even the most demanding separations of samples containing complex mixtures of polar/non-polar, hydrophilic/hydrophobic substances. Even chiral substances are resolved with suitable chiral HPCPCTM phases. Work at any pHWith HPCPCTM, there is no need to concern yourself with unwieldy pH limitations that are often associated with solid stationary phase supports. Innovations overcome previous weakness of
the CPCWeak Point (1) Separation tasks are intricate and need to attend all the time. Overcome the above by innovated automation system. 1) Separation tasks can be preset on the 10 executed files. 2) Capable to link multiple files which have different separation conditions among the separation executed files (solvents, rotations, flow rates, etc.) so that the most suitable conditions can be considered sequentially. 3) Sequential separations with same executed file and sequential injections into same stationary phase (reproducibility is very good by means of using same condition for separation of samples) can be performed easily. Automation can be materialized by connection of Autosampler and Fraction Collector. 4) Data and file handlings will become easy by linking with computer in which data acquisition program was pre-installed. Weak Point (2) Take long time to decide the most suitable separation conditions. Overcome by improved small rotor which is just 1/3 volume of the previous model LLB-M.
|
|
| The Sanki HPCPCTM is a new liquid chromatographic technique
that utilizes liquid-liquid partition,
counter
current distribution, in the absence
of a
solid support, to perform separations
of
complex mixtures of chemical substances.
The HPCPCTM is an alternative to packed-bed columns
for preparative HPLC and operates by classical
liquid-liquid partitioning in a high performance
centrifugal system. A solid stationary phase
is not used. Instead, stationary-phase liquid
is retained by centrifugal force in discrete
partition channels within a unique patented
circular Partition Disk Pack. A packed column
generally contains only 2 to 7 percent of
stationary phase, severely limits its capacity.
In an HPCPCTM system, the column contains between
50 and 80 percent stationary phase. The stationary
phase is held in numerous discrete partition
cells. Microdroplets of mobile phase liquid
pass continuously through the stationary
phase liquid. Any two-phase solvent mixture
can be used, at any pH, to perform normal
and reversed phase chromatographic separations. An injected sample, carried by the mobile phase, moves sequentially through the partition channels, where components are partitioned between the mobile and stationary liquid phases, separated from each other on the basis of differences in their partition coefficients, and eluted. |
Simple scheme used by the pioneers of CPC
to represent the arrangement between channels
and ducts, with the droplets of mobile phase
flowing in the stationary phase. |
Schematic representatin of the CPC apparatus. |
 |
| [Recommended reference : Centrifugal Partition Chromatography edited by Dr. Alain P. Foucault.] |
|
Single HPCPCTM Unit Introduces Dual Applications
| Separation by either normal-phase or reversed-phase elution is accomplished with a single two-phase solvent system. Dual-mode HPCPCTM is illustrated for a hypothetical mixture of five components (a, b, c, d and e) as below. Assuming their "partition coefficients" (ratio of concentrations in stationary / mobile phase) are in the order a>b>c>d>e. |
| (1) | Normal Phase Separation | (2) | Normal Phase Elution |
 |
 |
||
| (1) (2) |
The components with larger partition coefficients
(a & b) are primarily remained
in the
"Lower Stationary Phase"
due to
their strong affinity for the Lower. The components with smaller partition coefficients (c, d & e) are separated from each other via "Upper Mobile Phase Elution". During upper phase elution, however, components a and b may migrate, very slowly and actually separated from each other within the stationary phase. |
| (3) | Recycle | (4) | Reversed Phase Elution |
 |
 |
||
| (3) (4) |
After c, d & e have eluted, the Upper
Mobile Phase is recirculated for a
time sufficient
for compete partition of components
a &
b. The phases and the flow direction are reversed at this point and components a & b are eluted via "Lower Phase Elution". |
|
 |
1) | Washing |
 |
||
| 2) | Injection | |
 |
||
| 3) | Ascending Mode (Mobile Phase : Upper) | |
 |
||
 |
4) | Descending Model (Mobile Phase : Lower) |
 |
||
 |
||
 |
||
|
|
Alkaloids (1-Strychnine, Quinine, Cinchonidine) |
|
Selection of Two-Phase Partition Solvents
| A two-phase solvent system is used as separation medium in Centrifugal Partition Chromatography. One serves as the stationary phase, the other serves as the mobile phase. The solvents may be selected from an infinite variety of possible combinations. Followings are some frequently used solvent combinations. |
 |
