Separation of Alkaloids 1-Strychnine, Quinine and Cinchonidine

Operation Condition
Two Phase : Mobile phase : Sample : Rotation : Flow speed : Detection :	Chloroform : Methanol : 0.6M hydrochloric acid = 4 : 4 : 3 Bottom (normal phase, 675 ml) Top (reversed phase, 125 ml) 5 mg each 500 rpm 4.5 ml/min UV 254 nm